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Fig. 6. (A) Western blot analyses of Bax, Bcl-2, PCNA and caspase-3 in testes of adult mice offspring at PND 75 of lactating dams who were exposed to PTU and BDE-209 (500 and 700 mg/kg BW) in comparison to vehicle- treated controls. All protein expression levels were normalized relative to α-tubulin loading control. (B) The densitometric data of Bax, Bcl- 2, PCNA and caspase-3 are presented as the mean of integrated relative density value (IRDV)±S.E.M. of triplicate blots. Representa tive testicular sections showing <t>TUNEL-positive</t> cells (arrows) in seminiferous tubules in adult mice testes of lactating dams who were exposed to PTU (C), vehicle (D) and BDE-209 (E: 500 mg/kg BW; F: 700 mg/kg BW). Scale bar: 50 µm (C-F). (G) Histogram shows an increase in the number of TUNEL-positive cells in testes of PTU and BDE-209-exposed offspring at PND 75. Values are mean ±S.E.M. *Significantly different from controls (p < 0.05) by ANOVA followed by Newman- Keuls’multiple range test.
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Fig. 6. (A) Western blot analyses of Bax, Bcl-2, PCNA and caspase-3 in testes of adult mice offspring at PND 75 of lactating dams who were exposed to PTU and BDE-209 (500 and 700 mg/kg BW) in comparison to vehicle- treated controls. All protein expression levels were normalized relative to α-tubulin loading control. (B) The densitometric data of Bax, Bcl- 2, PCNA and caspase-3 are presented as the mean of integrated relative density value (IRDV)±S.E.M. of triplicate blots. Representa tive testicular sections showing <t>TUNEL-positive</t> cells (arrows) in seminiferous tubules in adult mice testes of lactating dams who were exposed to PTU (C), vehicle (D) and BDE-209 (E: 500 mg/kg BW; F: 700 mg/kg BW). Scale bar: 50 µm (C-F). (G) Histogram shows an increase in the number of TUNEL-positive cells in testes of PTU and BDE-209-exposed offspring at PND 75. Values are mean ±S.E.M. *Significantly different from controls (p < 0.05) by ANOVA followed by Newman- Keuls’multiple range test.
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Fig. 6. (A) Western blot analyses of Bax, Bcl-2, PCNA and caspase-3 in testes of adult mice offspring at PND 75 of lactating dams who were exposed to PTU and BDE-209 (500 and 700 mg/kg BW) in comparison to vehicle- treated controls. All protein expression levels were normalized relative to α-tubulin loading control. (B) The densitometric data of Bax, Bcl- 2, PCNA and caspase-3 are presented as the mean of integrated relative density value (IRDV)±S.E.M. of triplicate blots. Representa tive testicular sections showing <t>TUNEL-positive</t> cells (arrows) in seminiferous tubules in adult mice testes of lactating dams who were exposed to PTU (C), vehicle (D) and BDE-209 (E: 500 mg/kg BW; F: 700 mg/kg BW). Scale bar: 50 µm (C-F). (G) Histogram shows an increase in the number of TUNEL-positive cells in testes of PTU and BDE-209-exposed offspring at PND 75. Values are mean ±S.E.M. *Significantly different from controls (p < 0.05) by ANOVA followed by Newman- Keuls’multiple range test.
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Fig. 6. (A) Western blot analyses of Bax, Bcl-2, PCNA and caspase-3 in testes of adult mice offspring at PND 75 of lactating dams who were exposed to PTU and BDE-209 (500 and 700 mg/kg BW) in comparison to vehicle- treated controls. All protein expression levels were normalized relative to α-tubulin loading control. (B) The densitometric data of Bax, Bcl- 2, PCNA and caspase-3 are presented as the mean of integrated relative density value (IRDV)±S.E.M. of triplicate blots. Representa tive testicular sections showing <t>TUNEL-positive</t> cells (arrows) in seminiferous tubules in adult mice testes of lactating dams who were exposed to PTU (C), vehicle (D) and BDE-209 (E: 500 mg/kg BW; F: 700 mg/kg BW). Scale bar: 50 µm (C-F). (G) Histogram shows an increase in the number of TUNEL-positive cells in testes of PTU and BDE-209-exposed offspring at PND 75. Values are mean ±S.E.M. *Significantly different from controls (p < 0.05) by ANOVA followed by Newman- Keuls’multiple range test.
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Fig. 6. (A) Western blot analyses of Bax, Bcl-2, PCNA and caspase-3 in testes of adult mice offspring at PND 75 of lactating dams who were exposed to PTU and BDE-209 (500 and 700 mg/kg BW) in comparison to vehicle- treated controls. All protein expression levels were normalized relative to α-tubulin loading control. (B) The densitometric data of Bax, Bcl- 2, PCNA and caspase-3 are presented as the mean of integrated relative density value (IRDV)±S.E.M. of triplicate blots. Representa tive testicular sections showing TUNEL-positive cells (arrows) in seminiferous tubules in adult mice testes of lactating dams who were exposed to PTU (C), vehicle (D) and BDE-209 (E: 500 mg/kg BW; F: 700 mg/kg BW). Scale bar: 50 µm (C-F). (G) Histogram shows an increase in the number of TUNEL-positive cells in testes of PTU and BDE-209-exposed offspring at PND 75. Values are mean ±S.E.M. *Significantly different from controls (p < 0.05) by ANOVA followed by Newman- Keuls’multiple range test.

Journal: Ecotoxicology and environmental safety

Article Title: Decabromodiphenyl ether (BDE-209) exposure to lactating mice perturbs steroidogenesis and spermatogenesis in adult male offspring.

doi: 10.1016/j.ecoenv.2020.111783

Figure Lengend Snippet: Fig. 6. (A) Western blot analyses of Bax, Bcl-2, PCNA and caspase-3 in testes of adult mice offspring at PND 75 of lactating dams who were exposed to PTU and BDE-209 (500 and 700 mg/kg BW) in comparison to vehicle- treated controls. All protein expression levels were normalized relative to α-tubulin loading control. (B) The densitometric data of Bax, Bcl- 2, PCNA and caspase-3 are presented as the mean of integrated relative density value (IRDV)±S.E.M. of triplicate blots. Representa tive testicular sections showing TUNEL-positive cells (arrows) in seminiferous tubules in adult mice testes of lactating dams who were exposed to PTU (C), vehicle (D) and BDE-209 (E: 500 mg/kg BW; F: 700 mg/kg BW). Scale bar: 50 µm (C-F). (G) Histogram shows an increase in the number of TUNEL-positive cells in testes of PTU and BDE-209-exposed offspring at PND 75. Values are mean ±S.E.M. *Significantly different from controls (p < 0.05) by ANOVA followed by Newman- Keuls’multiple range test.

Article Snippet: TdTmediated dUTP nick end labeling (TUNEL) assay kit (4810–30-CK) was purchased from R&D system Inc., Minneapolis, USA.

Techniques: Western Blot, Comparison, Expressing, Control, TUNEL Assay